Academy Project: Investigating feasibility of scaling up crystallization of a membrane protein

Aquaporin A/S is a pioneering biotechnology company at the forefront of water purification and filtration. By harnessing the natural water channels present in living organisms, Aquaporin specializes in the development of cutting-edge membrane technologies that facilitate highly efficient water treatment processes. At the heart of its innovation lies the Deep Tech Center, where research is dedicated to the advancement of the next generation of biomimetic membranes. This next phase focuses on every aspect of biomimetic membrane development, from protein production to protein stabilization and integration into the filtration membrane. Aquaporin's commitment to pushing the boundaries of biomimicry promises to revolutionize water purification, offering sustainable solutions to address pressing global water challenges.

Objective

The primary objective of this project is to investigate the feasibility of scaling up a protein stabilization technique for the crystallization of membrane proteins in 2D sheets. This ambitious endeavor requires a significant increase in volume, alteration of chemicals, and innovative utilization of equipment. Through a comprehensive experimental and theoretical study, the aim is to assess the viability of scaling up this crucial process, which holds immense potential for advancing biomimetic membrane development.

Tasks

Literature study on membrane proteins, 2D sheet formation, and detergent study.

Experimental tasks:

• Volume Scalability Assessment: Determine the optimal scale-up factors to maintain protein integrity and sheet morphology.
• Chemical Modification and Compatibility Testing: Investigate the impact of altering chemicals and concentrations on the stability and ability to form crystals of membrane proteins. Identify suitable chemical formulations that facilitate scalability without compromising the structural integrity of the 2D sheets.
• Equipment Innovation and Optimization: Explore innovative approaches to adapt existing laboratory equipment and develop a setup capable of accommodating larger volumes and facilitating the formation of uniform 2D protein sheets. Optimize equipment performance to ensure reproducibility and scalability across different experimental conditions.
• Characterization: Employ advanced analytical techniques, such as transmission electron microscopy (TEM) to characterize the morphology and structure of the scaled-up 2D protein sheets.

Student Opportunities and Profile

This project offers a student an opportunity to engage in a multifaceted learning experience that combines theoretical exploration with hands-on experimental work. The student will have the freedom to exercise their creativity by designing experimental setups and analysis methods, allowing him/her to tailor his/hers approach to the specific objectives of the project. Through thorough training provided at the outset, the student will acquire the necessary skills and knowledge to work independently in the laboratory. This training encompasses both theoretical understanding and practical proficiency, ensuring that the student is equipped to tackle the challenges of the project with confidence. By actively participating in the design, execution, and analysis of experiments, the student will develop critical thinking skills, problem-solving abilities, and a deeper understanding of the subject matter. Overall, this project offers an enriching experience that empowers the student to take ownership of his/hers learning journey and make meaningful contributions to scientific research. Ideal candidates for this opportunity possess a strong interest in biotechnology or biochemistry, a passion for scientific inquiry, and a willingness to learn and explore new concepts and techniques.

Project Type

• Internship
• Special course
• Bachelo'rs Thesis, or
• Master's Thesis

Project timing

Autumn semester 2024, for a minimum of 3 months preferably 6 months, depending on the type of project.

All work will be conducted in a safe manner according to Danish safety standards.